Published Paper Details:

One of the most diverse groups of vertebrate is teleosts. They use a wide range of reproductive methods. Vitellogenin molecules of different fish species have been widely studied for their role in fish reproduction. The protein fractions of selected ornamental fish have been analyzed in the present study. The ovary tissue samples of oviparous fish (goldfish and rosybarb) and viviparous fish (white molly and guppy) treated with natrum muriaticum exhibited different protein fractions on SDS-PAGE analysis. Results revealed that there are striking similarities between the protein fractions of ovary tissues. The analysis of polypeptides of oviparous fish by SDS-PAGE revealed the presence of nine fractions and eight fractions in viviparous fish. Three bands were more prominent in both categories. These are considered as specific bands for reproductive functions of oviparous and viviparous fish on the observations made in ornamental fish. The variation in their number and staining intensities of different fractions may reflect their different type of metabolic activity, reproductive age, and their environmental conditions. The result showed that the synthesis of proteins during natrum muriaticum exposure in ornamental fish signifies that and these proteins are female specific proteins. These proteins are involved in vitellogenesis. However, further experiments are needed to sequence the whole vitellogenin at gene level.