Published Paper Details:

Nano liquid chromatography is a kind of microfluidic system used to resolve different pharmaceutical, clinical, biomedical, chiral, metabolic, protein, peptides and enantiomeric compound. It is used to detect compound in nanogram level by using nano liter of solvents hence ensuring low consumption of solvent. Miniaturized separation techniques have emerged as ecofriendly alternatives to available separation methods. Nano-liquid chromatography (nano-LC), microchip devices and nano-capillary electrophoresis are miniaturized techniques that minimize reagent utilization and waste generation. Moreover, high cost of equipment may be some limitation although nano liquid chromatography is a newer technology ensures opening of new bridge to newer application for industry and laboratories. This article reviews the instrumentation used for nano-flow LC, the types of columns employed, and techniques for multidimensionality of separations, which might be key to the future state of the technique to the high-throughput needs of modern bioanalysis. Some theoretical aspects are explained to better explain both the potential and the principal limitations of nano-LC. An update of the current applications where nano-LC is widely used, such as proteomics and metabolomics, is discussed. Recent pharmaceutical and biomedical applications of this separation methods are also presented to signify the high-quality satisfactory performance for complex matrices, especially for proteomic analysis, that is achieve with nano-LC.