Published Paper Details:

Protein quantification is necessary to understand the total protein content in a sample or in a formulated product. Accurate protein quantification is important as a range of other critical assays require precise total protein content results in order to generate data. The estimation of protein is done for four different plants Carica papaya, Ananas cosmosus, Wrightia tinctori,Milletia pinnata. Determination of protein concentration is necessary and widely used in protein biology, molecular biology, and other research applications. The concentration of protein samples have to be estimated before proceeding to isolation, purification, and analysis. The Bradford protein assay which was developed by Marion. M. it is a quick and accurate spectroscopic analytical procedure used to measure the concentration of protein in a solution. The reaction is dependent on the amino acid composition of the measured proteins. The protein content of pineapple leaves is found to be the highest whereas the lowest protein content is found in pongamia.