Published Paper Details:

An easiest, precise, accurate and isocratic RP-HPLC stability-indicating method was developed and validated for determination of Beta caryophyllene. RP-HPLC separation was achieved on Hypersil BDS RP (5?, 250 mm X 4.6 mm) column using mobile phase comported of methanol: water: orthophosphoric acid (98:02:0.1, % v/v) at flow rate of 1 ml/ min. Forced declination studies were performed on bulk sample of Beta caryophyllene using acid (1 N Hydrochloric acid), base (1 N Sodium hydroxide solution), oxidation (3% Hydrogen Peroxide Solution), Dry heat (80?C). Good resolution between the peaks corresponds to declination products. The estimation angles of Beta caryophyllene showed good linearity in the attention range 25-75 ?g/ ml with UV discovery (210 nm). The correlation portions were in the range 0.999. With limit of discovery and quantification 1.976 ug/ml and 5.989 ?g/ml, independently. The system has the needful delicacy, selectivity, perceptivity and perfection to assay declination products performing from the stress studies didn't intrude with assay is therefore stability- indicating.