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Abstract Introduction: Organ donation is the practise of harvesting a person's organs after he or she has died in order to transplant them into another person. The procedure of medically taking an organ or tissue from one person (donor) and implanting it into another person is known as organ donation (OD) (recipient). Objectives: To assess the effect of awareness on knowledge and attitude towards organ donation among undergraduate nursing students. Methodology: Quantitative approach with quasi experimental design was used & samples were final year B.Sc. nursing students selected through non-probability convenient sampling. Data has collected through structured questionnaire to assess knowledge, Likert scale to assess attitude. Pilot study followed by actual data collection was done & analyzed using descriptive & inferential statistics. Result: Statistical analysis shows that the pre-test mean knowledge score is 7.6 whereas the mean score is 11.76. Paired t test gives t values 0.05 that has high level of significant as p=<0.05. The mean difference in pertest & postest knowledge mean score was 4.16 in the experimental group whereas in control group was 0.88. Paired t test indicates that the difference in the mean score of experimental groups was statically significant than the control group with the p value i.e. p <0.05 in the experimental group. Significant difference in the attitude mean score of both the groups i.e., for positive attitude 10.6 in experimental group & 0.32 in the control group. Also, for negative attitude mean score 11.48 in experimental group & 0.88 in control group. A greater difference in mean score was observed in the experimental group. Paired t test indicates that difference in the mean score of experimental groups was statistically significant than the control group with p value that is p=0.05 is experimental group. For Positive attitude, in experimental group, 6(24%) students had favorable attitude & 14(56%) had Neutral attitude & 5(20%) students had unfavorable attitude during pretest whereas 24(96%) students had favorable attitude & 1(4%) had Neutral attitude during post test towards Organ donation. In Control group, 7(28%) students had favorable attitude & 8(32%) had Neutral attitude & 10(40%) students had unfavorable attitude during pretest whereas 8(32%) students had favorable attitude & 7(28%) had Neutral attitude &10(40%) had unfavorable attitude towards Organ donation during post test towards Organ donation. for Negative attitude, in experimental group 7(28%) students had favorable attitude & 13(52%) had Neutral attitude & 5(20%) students had unfavorable attitude during pretest in experimental group whereas 25(100%) students had favorable attitude during post-test in towards Organ donation. Whereas in Control group, 4(16%) students had favorable attitude & 13(52%) had Neutral attitude & 8(32%) students had unfavorable attitude during pretest whereas 4(16%) students had favorable attitude & 15(60%) had Neutral attitude& 6(24%) students had unfavorable attitude during post-test towards Organ donation. Hence awareness was more effective in improving knowledge and attitude towards organ donation.