Published Paper Details:

Toxic effects of fungicide, MAJOR-75 were evaluated by analyzing root growth and root morphology. The higher concentration (500 ppm) of fungicide an inhibition of root growth and there was a statistically significant difference between control groups. In addition, cyto- and genotoxicity were estimated by observing cytological parameters such as the mitotic index and number of chromosome abnormalities, including chromosome breaks, stickiness, and polar deviations. The mitotic index (MI) of A. cepa meristematic cells treated with the 500 ppm of fungicide was significantly decreased in comparison to the negative control. The obtained results indicate that MAJOR-75 had the ability to cause a reduction in the seed germination percentage in the number of different phases of mitosis. The fungicide treatment in root meristem cells of A.cepa with different concentrations resulted in the reduction in morphological and mitotic frequency of root tip cells of Onion. As we know, mitotic frequency reflects cell division frequency and is used to determine the root growth ratio as a significant parameter. Regardless of which process is affected, both processes result in a reduced supply of new cells in the root meristem leading to an eventual inhibition of growth.