Published Paper Details:

The present study aimed to examine the comparative study on the amelioration of cypermethrin hepatic toxicity by aqueous extract on Annona muricata leaf and methotrexate in albino rats. Wistar strain albino rats of 130-250 grams of body weight (BW) were selected and divided six groups of five rats. First group (Group I) served as normal control. Second group (Group II) given cypermethrin (CYP) (0.05 ml/100g BW/day) for 60 days. Third group (Group III) given aqueous Annona muricata leaf extract (AMLE) (0.05 ml/100g BW/day) for 60 days orally. Fourth group (Group IV) was treated with Methotrexate (MTX) (1.0mg/100 gm BW/day) once a week intraperitoneally. Fifth and Sixth group (Group V & Group VI) given cypermethrin (0.05 ml), with supplementations of AMLE (0.05 ml) and methotrexate (1.0 mg/ 100gm BW/week) resp. for 60 days. A significant increase in body weight can be seen in all treatment groups expect MTX treated group, when compared to control, the rate of increase is observed to be almost equal irrespective of treatment on comparison with cypermethrin treated control. On comparison with control a significant decrease in liver weight can be observed in all the treatment groups except MTX supplementation group. But no significant changes can be observed on AMLE supplementation, while MTX supplementation seems to be more beneficial in liver weight. Control group showed normal hepatocytes in trabeculae separated by sinusoids. while no significant pathological changes were observed on AMLE treatment, CYP as well as MTX brought about an alteration in lobular architecture. Concurrent supplementation was AMLE showed altered lobular architecture with vacuolations and necrosis in hepatocytes, while MTX supplementation showed normal lobular architecture with mild interface hepatitis.