Published Paper Details:

The molecular data, in particular gel electrophoresis of enzymes and numerical methods of analysis have proven useful in many groups of insects and will see much wider use in future. Therefore, present study was designed with the main purpose to analyze the activities of alkaline phosphatase isozymes by electrophoresis method during different developmental stages of newly evolved races R1 and R2 of Bombyx mori L. Standardized disc electrophoresis method was performed. Alkaline phosphatase isozymes form distinct enzymes zones in the photographs and in the zymogram and these have been numbered in cathodal to anodal sequence. These isozyme patterns have been established after repeated runs. The total isozymes of different developmental stages of R1 and R2 have been grouped into different zones. The nomenclature of enzyme banding pattern has been followed. The relative front (Rf) of the esterase and phosphatase bands of all the developmental stages with reference to known indicator dye was calculated. Results demonstrated that changes in APH zymograms during development stages revealed a total of 18 bands R1, and 16 bands in R2. From the present results it is clear that APH the activity in eggs was low in R1 and R2. In the larval stage, APH activity was higher in R1, and in R2 moderate activity was exhibited in larval as well pupal stages. In pupal stage the activity was less in male pupae of R1 & R2, and it was found to be high in female pupae R1 & R2.