Published Paper Details:

RP-HPLC method was developed for the estimation of Candesartan cilexetil and Hydrochlorothiazide in tablet dosage form with the help of Quality by Design (QbD) approaches. In this method concentration of each drug was obtained by using the absorptivity values calculated for drug wavelength 262 nm and solving the equation. The RP-HPLC method was performed C18 (250mm x 4.6 mm,) 2.5 ?m particle size in gradient mode, and the sample was analysed using Acetonitrile 25.0 ml and 75.0 ml (pH 2.7 0.1% OPA with water) as a mobile phase at a flow rate of 0.7 ml/min and detection at nm. By the retention time for Candesartan cilexetil and Hydrochlorothiazide found 3.046 and 6.161 min respectively. Validation related the method is specific, rapid, accurate, precise, reliable, and reproducible. Calibration plots by both HPLC were linear over the 16-80 and12.5-62.5 ?g/ml for Candesartan and Hydrochlorothiazide respectively, and recoveries from tablet dosage form were between 99.02 and 100.00 %. The method can be used for routine of the quality control in pharmaceuticals. The degradation profiling of Candesartan and Hydrochlorothiazide were also carried out.