Published Paper Details:

We have here studied some conventional and novel method approaches for extraction of Mangiferin from leaves of Mangifera Indica (Mango). The one of the common methods used for extraction of Mangiferin is three phase partitioning. The other methods such as Batch extraction, Soxhlet extraction were also studied to see which method gives the optimum yield of Mangiferin. Three phase partitioning (TPP) is the method of extraction in which two phases viz. organic phase and aqueous phase are formed by salt (mostly Ammonium sulphate) saturation, continuous addition of solvent (mostly t-butanol) and adjusting the pH respectively. TPP is the most simple, economical and quick method for recovering Mangiferin from Mangifera Indica leaves as it requires less amount of solvent for extraction and also takes less time. Various parameters which have effects on TPP extraction of Mangiferin such as right salt selection, extraction time, slurry to t-butanol ratio, solute to solvent ratio and right pH were tested to get the optimum yield of Mangiferin. In the study, it was observed that in Soxhlet extraction, the mangiferin content was found to be 57mg/g in 5 hr. time, while in TPP the mangiferin content was 28mg/g in 2 hr. time. Also, conventional batch extraction using water as solvent gave the total yield of 23 mg/g in 2 h 40 min.