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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
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Paper Title
DEVELOPMENT OF AN ELISA BASED DETECTION KIT USING CHICKEN EGG YOLK ANTIBODY AGAINST GASTEROINTESTINAL INFECTION CAUSING SALMONELLA ENTERITIDIS IN POULTRY
Authors
Dr.Mahenthiran.R, Vishnu priyanka.K
Keywords
Keywords: Gastro-intestinal infection, Salmonella enteritidis, IgY, ELISA, SDS-PAGE, Micro agglutination, Cross reactivity, ELISA kit
Abstract
Abstract: Salmonella enteritidis is a serotype of Salmonella enterica which causes Non-typhoidal and Gastro-intestinal infection in both humans and animals. Chicken IgY has been identified as an alternative for the treatment of non-typhoidal salmonellosis. 21 weeks old white leghorn chicken which lays eggs was immunized with Salmonella enteritidis. The anti-Salmonella enteritidis IgY was purified and their protein content was estimated. The specificity of produced IgY was tested with Rapid Agglutination test, Micro agglutination test and Cross reactivity test. SDS-PAGE analysis of IgY showed a protein profile of the IgY, antibody titer potency of each serum and IgY was determined by Indirect ELISA. The present study was conducted to develop both Direct and Indirect ELISA kit for the detection of Salmonella enteritidis infection.
IJCRT's Publication Details
Unique Identification Number - IJCRT2105412
Paper ID - 207124
Page Number(s) - d657-d667
Pubished in - Volume 9 | Issue 5 | May 2021
DOI (Digital Object Identifier) -
Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
Dr.Mahenthiran.R, Vishnu priyanka.K, "DEVELOPMENT OF AN ELISA BASED DETECTION KIT USING CHICKEN EGG YOLK ANTIBODY AGAINST GASTEROINTESTINAL INFECTION CAUSING SALMONELLA ENTERITIDIS IN POULTRY", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.9, Issue 5, pp.d657-d667, May 2021, Available at :http://www.ijcrt.org/papers/IJCRT2105412.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
ISSN and 7.97 Impact Factor Details
ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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