Published Paper Details:

Today Abiotic stress is a major global problem limiting crop productivity and Stress factors are a serious problem limiting the yield potential of modern cultivars faced by mankind now a days. Heavy metals represent a growing threat for ecosystems worldwide. In India, several studies have searched the amounts of heavy metals in soils at roadsides of high ways and soils of litter dumps. The conducted studies found that these soils contained worrying levels of heavy metals that have exceeded in many cases the average world safe limit. For example, Chromium (Cr) level was 79 mg/ kg in soils closed to some highways while the world safe limit is only (25 mg/kg). In other studies, Nickel (Ni) concentration was (5.9 mg/kg) while the world safe limit for Mn is only (0.03 mg/kg). Also in some litter dumps in India, soil Magnesium (Mg) average content was (6.0 mg/kg) while the world safe limit was only (0.1 mg/kg). Vigna mungo (L.) Hepper is a flowering plant that has recently attracted the attention of researchers due to its novel phytoremediation powers against some heavy metals, which encouraged some countries to grow them in roadsides of highways and litter dumps. Tissue culture is an excellent approach for studying responses of Vigna mungo (L.) Hepper to heavy metals without interference of other factors. In addition, tissue culture is the main method for producing elite plants lines from callus and cell suspension cultures with superior characteristics by genetic transformation. Stress causes nutritional imbalances in the plant causing reduction in water uptake and toxicity, decreasing the production and Seeds of Vigna mungo (L.) Hepper T-9 var were taken as cotyledonary explants here and inoculated on callus induction medium with varied concentration of MS+ 2.4-D (1.0-3.0mg/L). (2.5mg.L 2.4-D) Induced best callus induction. This calli were formed in treatments with (0.5, 1.0, 2.0, 3.0, 5.0, 7.5 and 10 mg/1) concentration of Mn, Cr, Ni and Mg. Callus induction and rhizogenesis was studied with incorporation of some heavy metals individually at different concentrations in MS medium supplemented with MS+ 2.4-D (2.0 mg/L) each. The study was carried out to see the overall effect of selected metals in vitro on variations in induction, nature and growth of callus and rhigogenesis produced in relation to metals and discussed in detail. The study was carried out to see the overall effect of selected metals in vitro on variations in induction, nature and growth of callus and rhigogenesis produced in relation to metals and discussed in detail.