Published Paper Details:

Now a days synthetic pyrethroids are widely available in the field of agricultural sector to achieve the highest yield. Among the pyrethroids bifethrin has good persistence and insecticidal activity. Bifethrin enters through different routes into aquatic organisms and then accumulate in various vital organs of fresh water fish Labeo rohita which ultimately reaches the humans through food chain and shows its adverse effects. SDH, MDH and LDH are the enzymes secreted in the organisms for the oxidation of glucose molecules and for the liberation of usable chemical energy in the form of ATP. In the present study test fish Labeo rohita was exposed to sub lethal concentrations of bifethrin for different exposure periods i.e for 24hrs, 5days and 10 days to evaluate the enzymatic activity of Succinate Dehydrogenase(SDH), Malate Dehydrogenase(MDH) enzymes which participate in aerobic respiration and Lactate Dehydrogenase (LDH) enzyme which participate in the anaerobic respiration in various organs of fish such as brain, gill, liver, kidney and muscle. In this case study the levels of the enzyme LDH was elevated due to the requirement of quick energy resource (ATP) for the organism in all exposure periods whereas the enzymatic activity of SDH and MDH were depleted in all the tissues of the experimental fish Labeo rohita because of the reason that it takes time to oxidize glucose molecule in aerobic respiration. Under the exposure of Bifethrin in Sub-lethal concentrations on the10thday maximum percentage of elevation of LDH was observed in liver(31.57%) and minimum percentage of elevation of LDH was observed in muscle (18.36%) . On 10th day , the maximum percentage of depletion of SDH was seen in kidney (-30.77%), minimum depletion of SDH was observed in brain (-16.90%), MDH level was maximum in liver (-22.78%) and minimum in kidney (-17.02%) was observed when all the exposures were compared with controls.