Published Paper Details:

ABSTRACT Breast cancer is the serious health concern in India causing the highest mortality rate in females, which occurs due to uncontrolled cell division and can be metastasize to other parts of the human body.Natural products are important leads in drug discovery. The search for effective plant-derived anticancer agents or their synthetic analogues has continued to be of interest to biologists and chemists for a long time. We investigated the, cytotoxicity and anticancer activity of new synthetic 4-Hydroxypropiophenone (4-HPPP) was tested against breast cancer cell line MCF-7. Cytotoxicity was examined using MTT assay. The ability to induce apoptosis and changes in mitochondrial membrane potential,levels of reactive oxygen species and comet Assay.Based on the results, we determined the effective dose of 4-HPPP as 100?M/ml for 24 hr. Cytotoxicity effects of 4-HPPP was confirmed by treatment of MCF-7cell with IC50 concentration of 4-HPPP resulted in sequences of events marked by the enhance the apoptosis accompanied by loss of cell viability, modulation of reactive oxygen species and cell cycle arrest through the induction of G0/G1 phase arrest on MCF-7cells. These findings show that the synthetic 4-HPPP, displays potential to be further explored in the development of new anticancer agents.