Published Paper Details:

NO3- and NH4+ are the common forms of nitrogen that all plants utilize for proper growth and development. Nitrogen significantly increases and enhances the yield and its quality by playing a vital role in biochemical and physiological functions of the plant. Both form and amount of nitrogen in nutrient medium have significant effects on the rate of cell growth and differentiation. Uniqueness of nitrogen lies in the fact that plants can use it either in anionic (NO3-) or in cationic form (NH4+). This paper examines the present knowledge of the main effects that different concentrations of nitrogen have on plant morphogenesis in vitro. During the present study, effect of different concentrations of nitrogen on in vitro morphogenesis of Petunia hybrida Hort. (Vilm) was evaluated. In the present study it was concluded that maximum callus production and root formation was observed in MS Basal Medium and the medium containing half concentration of nitrogen.