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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
Call For Paper - Volume 12 | Issue 5 | Month- May 2024
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Published Paper Details:
Paper Title
MOLECULAR CLONING OF AN α-AMYLASE GENE FROM BACILLUS subtilis AND IT`S EXPRESSION ON E.coli
Authors
Saurabh Singh, Diljan Khan, Rashmi Jaiswal
Keywords
?-amylase, Fermentation, Transformation, cloning
Abstract
Amylase are among the most important enzymes and are of great significant for the field of food industry, constituting a class of industrial enzymes having approximately 25% of the world enzyme market. Alpha-amylase have potential application in a wide no. of industrial process such as food, fermentation, textile, paper, detergent and pharmaceutical industries. In this present study we have isolated alpha-amylase producing gene (Alf A gene) from Bacillus subtilis ATCC6051, then this Alf A gene is ligated in T&A plasmid then these plasmid are used in transformation. The transformed bacteria are selected by antibiotic containing plate. These transformed bacteria are checked for the production of ?-amylase enzyme by starch hydrolysis test.
IJCRT's Publication Details
Unique Identification Number - IJCRT2102062
Paper ID - 202967
Page Number(s) - 507-510
Pubished in - Volume 9 | Issue 2 | February 2021
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Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
Saurabh Singh, Diljan Khan, Rashmi Jaiswal, "MOLECULAR CLONING OF AN α-AMYLASE GENE FROM BACILLUS subtilis AND IT`S EXPRESSION ON E.coli", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.9, Issue 2, pp.507-510, February 2021, Available at :http://www.ijcrt.org/papers/IJCRT2102062.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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