Published Paper Details:

Abstract Shiitake mushroom Lentinula edodes (Berk.) Pegler is one of the most popular mushrooms for human consumption; it is a wood rot fungus capable of destroying the structural components of cellulose and lignin, and has several medicinal properties. This study was conducted to evaluate the local grain substrate, namely rice, corn, bajra, sorghum, wheat, for seed production using the small strain DMR 388. The results showed that the maximum mycelium growth on PDA medium was observed in the strain (89.4 mm) on the 20th day after inoculation, while minimal mycelium growth was observed in the wheat extract medium (72.50 mm). Among the 6 different substrates used for spawning production, the fastest mycelium growth was observed in paddy and wheat grains (31, 34.60 days, respectively), while the largest number of days was obtained in bajra (44.00 days) for the small strain DMR 388. In another experiment, various agricultural waste products such as wheat straw, paddy straw, corn straw, corn shelled cobs, sugarcane bagasse and saw dust with combination of corn shelled cobs were tested as substrates for indoor growing shiitake mushrooms using small strain DMR 388. Rapid mycelium growth was found on substrate corn shelled cobs (55.4 days). But mycelium growth was very slow on corn straw substrate (84.4 days). The yield attribute is not displayed because the fruiting body has not formed due to physical influences such as temperature, humidity, etc.