Published Paper Details:

Abstract: Caesalpinia bonducella (L.) Roxb. is a perennial climber belonging to the family Caesalpniaceae. The present study deals with the quantification of a unique homoisoflavanoid in the various plant organs and developmental stages of Caesalpinia bonducella (L.) Roxb. The methanolic extracts of various plant organs of Caesalpinia bonducella (L.) Roxb. viz root, stem, leaf, flower, pod, seed coat and seed kernel were subjected to HPLC analysis for the quantification of bonducellin. The bonducellin was present in flower, seed coat and seed kernel. The seed coat and seed kernel showed 0.001 � 0.31 �g/g DW and 0.004 � 0.27 �g/g DW. Maximum amount of bonducellin 1.834 � 0.04 �g/g DW was reported in the flowers of Caesalpinia bonducella (L) Roxb. The bonducellin was absent in other plant organs. The methanolic extracts of various developmental stages of Caesalpinia bonducella (L.) Roxb. viz 30 days old seedling plants, 60 days old seedling plants, 90 days old seedling plants, 120 days of the seedling plants, shoot before flowering, shoot at flowering and shoot after flowering were subjected to HPLC for the quantification of bonducellin. The amount of bonducellin in 30 days old seedling plants was 0.003 � 0.06 �g/g DW While it was 0.002 � 0.74 �g/g DW in 60 days old seedling plants. The seedling plants of 90 days old produced 0.001 � 0.32 �g/g DW while it was 0.002 � 0.93 �g/g DW in 120 days old seedling plants. The extract of shoot before flowering did not showed the presence of bonducellin. The shoot extract of plant at flowering and after flowering showed 0.013 � 0.40 �g/g DW and 0.013 � 0.40 �g/g DW bonducellin respectively. This study reveals the suitable plant organs and developmental stages of the plant to be used for the extraction of bonducellin which imparts many pharmacological activities of the plant.