Published Paper Details:

Brown seaweed Padina tetrastromatica is chief constituents of nutraceutical food and are regularly consumed by the Asian countries due to their large health benefits. Since earlier studies had been proved that P. tetrastromatica have pharmacological activities like antibacterial, antimicrobial, antifungal, antidiabetics, and cytotoxic activity. The present study was aimed to screen antioxidant activity of flavonoid rich fraction of P. tetrastromatica (FRPD). Extraction was done by maceration for 48 hours using methanol by modern sophisticated instruments. These extract was made into flavonoid rich fraction by partitioning with petroleum ether (60-800C) and n- hexane. The flavonoid rich extract was confirmed by phytochemical screening and the flavonoid contents are quantified by total phenolic and flavonoid content estimation. The total flavonoid and total phenolic content of the obtained fraction was found to be 174.71mg of quercetin equivalent, 246.28 mg GAE/g respectively. Antioxidant efficacies are also screened by DPPH and H2O2 radical scavenging assay and the total antioxidant capacity was also performed. The IC50 value of the antioxidant activity was found to be 121.92 ?g/ml, 254.73 ?g/ml, 190.99 ?g/ml respectively. This preliminary investigation might be useful to screen the plant in molecular levels.