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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
Call For Paper - Volume 12 | Issue 5 | Month- May 2024
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Paper Title
PROTEASES OF GERMINATING OF GREEN GRAM (VIGNA RADIATA) AND BLACK CHANA (CICER ARIETINUM) : PURIFICATION AND CHARACTERIZATION OF AN ALKALINE PROTEASES
Authors
Samridhi Bajwa , Nitu Trehan
Keywords
Germinated seeds, enzyme activity, proteases
Abstract
The crude protease was extracted from germinated seeds of Vigna radiata and Cicer arietinum. Protease was purified from these germinated seeds. Protein content was estimated by using Lowry method. Qualitative analysis was done where casein hydrolysis was observed by forming clear zones. Quantitative analysis was carried out by using casein as substrate. Effects of temperature and pH was also examined in which optimum pH was 8 while optimum temperature was 50?. Partial purification of germinated seeds was executed by ammonium sulphate precipitation with high osmotic resistance.
IJCRT's Publication Details
Unique Identification Number - IJCRT2008406
Paper ID - 198287
Page Number(s) - 3428-3432
Pubished in - Volume 8 | Issue 8 | August 2020
DOI (Digital Object Identifier) - http://doi.one/10.1729/Journal.24363
Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
Samridhi Bajwa , Nitu Trehan , "PROTEASES OF GERMINATING OF GREEN GRAM (VIGNA RADIATA) AND BLACK CHANA (CICER ARIETINUM) : PURIFICATION AND CHARACTERIZATION OF AN ALKALINE PROTEASES", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.8, Issue 8, pp.3428-3432, August 2020, Available at :http://www.ijcrt.org/papers/IJCRT2008406.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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