Published Paper Details:

DNA barcoding is a modern and broadly used molecular-based identification system that aims to identify biological specimens, and to allocate them to a given species. Though, DNA barcoding is even more than this, and moreover many practical uses, it can be measured the core of an integrated taxonomic system, where bioinformatics plays a key role. DNA barcoding data could be interpreted in diverse ways depending on the examined taxa but the technique relies on standardized approaches, methods and analyses. We tested two medicinal endangered plants Tylophora rotundifolia Buch.-Ham. ex Wight and Tylophora fasciculate Buch-Ham.ex Wight using two DNA barcoding regions (matK and rbcL). The matK showed poor results for both the plants and rbcL region showed good universality, and consequently the efficiency of these loci as DNA barcodes. We consequently tested the potentiality of the rbcl primer for the identification of two medicinal endangered species, which were collected from Shoolpaneshwar Sanctuary, Dediyapada and Gujarat. In this work rbcl primer were used to discriminate and confirm the identification of two medicinal endangered plants, it was found that, the potentiality of rbcL region in identification process for the two medicinal plants used is more efficiency than matK, where rbcl confirm the identification of two plants at species level.