Published Paper Details:

The main motive of the experiment was to investigate the efficiency of antioxidants in DNA damage repair when subjected to UV radiation. Main approach of the experiment was to investigate the concentration of the antioxidants that efficiently help in DNA damage repair. Method: Wild type E. coli as the experimenting organism and Vitamin-C as an antioxidant were taken. Both the E. coli was serially diluted by a factor of (10-1-10-5) dilution and Vit-C was taken in the concentration of (0�g/ml,45�g/ml,50�g/ml). Then the E. coli cultures grown with different concentrations of Vit-C were spotted on to LB media by Drop Plate Technique. The culture plates were subjected to the UV sensitivity assay by exposing to UV radiation at 254nm. Result: After the UV sensitivity assay, the culture plates were kept for overnight incubation. Incubated plates with 50�g/ml Vit-C and UV exposure, showed the maximum growth till 10-5 dilution factor, when compared with the control plate with UV exposure. The plates with 45�g/ml Vit-C with UV exposure, showed the growth till 10-4 dilution when compared with the control plate with UV exposure. The further analysis of the change in protein expression in analysed by SDS-PAGE. Conclusion: from the experiment we can conclude that Vit-C had efficiently reduced the cytotoxic effect of UV exposure by repairing the damaged DNA. It has left some scope of investigating the anti-cancer and anti-aging effects of Vit-C in the human system.