Published Paper Details:

The root system of higher plants is associated not only with an intimate environment composed of organic and inorganic substances but also with a vast community of metabolically active microorganisms. This paper investigates the enzyme characterization of bacteria from rhizosphere of Vitis vinifera. For the present work plant with high berry production was selected. Soil sample was collected aseptically from the rhizosphere, rhizoplane and non-rhizosphere, and different dilutions were made. Different selective media like Pseudomonas isolation agar, Minimal agar, Actinomycete isolation agar, Nutrient agar and Saborauds agar were used for isolation of rhizospheric microorganisms. Total 104 isolates of fungi and bacteria were isolated from rhizospheric soil and only 39 isolates were obtained from non- rhizosphere soil. These rhizospheric and non-rhizospheric isolates were characterized for enzyme production by using skim milk, chitin agar, xylan agar, tributyrine agar, starch agar, Pikovskaya medium, Czapek-mineral salt medium, Urea agar. The obtained results indicate that the isolated colony types like NAS I, MMS I, MMS III, MM1I, NAS V, NA1 III shows versatility in enzyme production. These types produce more than one types of enzymes. The obtained results indicate that the increase in berry production is due to the synthetic activity of rhizospheric microorganisms which helps in solubilisation of higher metabolic components to simple form so that they can be easily utilizable and will enhance plant growth and production.