Published Paper Details:

Regenerative medicine uses MSC as a tool to promote tissue regeneration. A major action of MSC to repair pathological degeneration is secreting paracrine secretory factors to reduce inflammation and accelerating tissue regeneration. MSC from different origins produce different significant trophic factors. Using MSC that fits to the need is a strategy for regenerative therapy. Here, we investigated paracrine bFGF TGF-? and IGF, as significant secretory factors, in MSC derived from human amniotic fluid (AF-MSC), Wharton�s jelly (WJ-MSC) and endometrium. The work quantified the levels of bFGF TGF-? and IGF gene transcriptions using quantitated real time RT-PCR and the secretory levels of trophic factors using ELISA assay. Our results indicated that bFGF was a significant trophic factor secreted by AF-MSC, whereas, TGF-? was a significant factor in WJ-MSC. AF-MSC secreted bFGF at 3 times over to control human fibroblasts. Secreting of IGF binding protein was found at a low level in AF-MSC, WJ-MSC and EM-MSC as compared to the secreting of control human fibroblast. Moreover, we found that WJ-MSC at late culture had a high level mRNA transcribed in bFGF TGF-? and IGF genes. They showed 2 times of bFGF and TGF-? genes expression and 7 times of IGF-1 gene expression higher than control. However, our study in allelic expression of IGF-2 imprinted gene analysis indicated epigenetic imbalance in WJ-MSC at late cultured passage. We conclude that an optimal MSC type for treatment in different pathologic diseases should be provided regarding a dissimilar significant trophic factor of each MSC origin. We also suggested that MSC in late cultured might great offer in regenerative medicine as cell-free therapy.