Published Paper Details:

Regenerative medicine is a strategy of treat degenerative pathology. Mesenchymal stem cell (MSC) has been used as tool for tissue regeneration. Both MSC embedding in scaffold and MSC from microfracture techniques are presented by previous study for regeneration at site of cartilage injury. To studied and compare cartilage regeneration by MSC embedding in scaffold and microfracture techniques, we contributed a cartilages defect rabbit knee using a stainless steel drill. PEGDA-scaffold, which was embedded with MSC was implanted over the left knee lesion, whereas, the injured right knee was drilled deep into subchondral bone for leaking of bone marrow-MSC. Rabbits were sacrificed at 12 weeks after surgery to examine macroscopic appearance of the articular following the modified ICRS evaluation. Macroscopic appearance was evaluated by immunohistochemistry. We found that cartilage repairs with the uses of microfracture technique showed a better result as compared to the uses of MSC/PEGDA scaffold implantation technique, as seen in ICRs scores. The treatment with microfracture technique improved smooth cartilage surface, subchondral bone formation and reduce inflammation in rabbit knee. We conclude that microfracture technique is an efficient treatment for tissue regeneration in knee injury.