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ABSTRACT Sum total of eighty samples were collected from Eleme and Port Harcourt for this study using forty (40) sterile swap sticks for each location. Two (2) different media (MacConkey Agar and Chocolate Agar) were used for the culture isolation of the microorganisms. Each swab stick was introduced on the surface of the media by streaking on the solid agar for microbial identification and biochemical analysis was done for specific identification of the microbes. Plasmid profiling of the isolated microorganisms to evaluate plasmid DNA was done by culturing in LB media with antibiotic selective pressure. This study identify four (4) microorganisms with Escherichia coli having the highest occurrence percentage of 46.30% from both location while 3.45% of Serratiam arcescen, 11.82% of Bacillus cereus and 38.43% of Staphylococcus aureus were isolated from both locations. The population of Escherichia coli isolated from Eleme and Port Harcourt was significantly high with percentage occurrence of 19.21% and 27.09 % respectively. This study implicated Escherichia coli and Staphylococcus aureus to be responsible for degradation of coated surface. Isolated from Eleme had plasmid of molecular weight 23.13kbp while Port Harcourt had plasmid of molecular weight 23.13kbp, and 4.361kbp showing significant change on their plasmid morphology.