Published Paper Details:

Rapid and high frequency in vitro multiple shoots and subsequent plantlets were developed in several plant species of arid forestry viz. Anogeissus acuminata, A.pendula, A. latifolia, A.sericea var. nummularia, A.sericea var. sericea, Calligonum polygonoides and Withania coagulans. In Prosopis cineraria, Tecomella undulata, Acacia senegal, Maytenus emerginata, Balanites aegyptiaca, Zizyphus nummularia and Z. mauritiana in vitro multiple shoots were induced at the high rate by using nodal shoot segments from mature trees, but root induction in differentiated shoots were not very high in few species. Dipcadi erythraeum - an endemic and endangered plant is differentiated into plantlet from in vitro cultures. Various explants (cotyledonary nodal segment and epicotyl of Anogeissus spp., nodal and apical shoot segments from mature plants) of 1.5-2.5 cm in length were placed on Murashige and Skoog's 1962(MS) basal medium supplemented with inorganic and organic addenda. Various growth regulators (auxins IAA, IBA, NAA and 2, 4-D and cytokinins; kinetin and BAP) were added from 0.1- 5.0 mg/l either alone or in combination to induce multiple shoots. Differentiated shoots could be further multiplied on fresh medium. Isolated shoots were rooted on MS basal and MS half strength basal salts medium with various concentrations of IAA, IBA and NAA. Plantlets of Anogeissus spp., Calligonum polygonoides, Withania coagulans and Dipcadi erythraeum were transferred into pots. . (N. S. Shekhawat et al. 1993,Rathore et al 1991,kaur et al 1992)