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Honeybees are well known to engage in highly preferential selection of the plant species whose flower they exploit for harvesting nectar, pollen or both at the same time. These are known to readily discriminate within various plants, selecting or tending to neglect others (Dhingra and Jain, 1995). Pollen is of vital importance to honeybees as it is the major source of nitrogenous food. Pollen is the ultimate source of carbohydrates, proteins, lipids and amino acids to all the species of family Apidae. Honeybees being Polylectic in nature are attracted to a variety of flowers. There have been so far many attempts to discover differences in pollen nutrients from various species. In most pollen, carbohydrates and proteins constitute the major matter. The nutritive value of pollen however in different plant species varies considerably (Singh, 1996). Honeybees also exhibit differences in feeding preferences and survival when fed pollen of different plant species (Schmidt et al, 1995). A few plant species even do not attract honeybees inspite of fact these yield sufficient pollen (Dhingra and Jain, 1995). Certain plants in the vicinity of agriculture crop to be pollinated by bees may thus prove more attractive to the bees; as a result many targeted crop will appear neglected for pollination. There has been many such studies reporting the problem of plant competition or identifying non-crop plants competing with beneficial crop for visit by pollen collecting honeybees (Olsen et al, 1979). Despite the fact that honeybees feed up on specific or a variety of pollen as food, there has been much been much difficulty in finding suitable substitute for pollen. Poor acceptability of pollen substituent may result in part due to lack of proper nutrients or the phagostimulants. Inspite of the fact that many reports are available on pollen composition of various bee preferred plant hosts as well as the bee collected pollens, information on what makes pollen most attractive or what pollen component particularly the amino acids contributes to pollen collection in honeybees is still obscure. Robinson and Nation (1968) concluded that pollen had a phagostimulatory effect on adult worker bees and suggested the presence of neutral lipids or free amino acids. Schmalzel (1980) has hypothesized that amino acids in pollen may act as phagostimulant alone or in concerned with the other substances for recoganization and pollen by Apis. Among the local flora, contain oil seed crops as toria (Brassica campestris L.), Indian mustard (Brassica juncea L.), sunflower (Helianthus annus L.) and some vegetable crops like radish (R.sativus.), onion (Allium cepa L.) etc. are of great importance to apiculturists. These crops exhibit differential attractivility for pollen collection to honeybees (Usha and Jain, 1996). It thus becomes imperative to make a qualitative analysis of the phagostimulatory effects of some of major pollen components of these hosts. This study was thus aimed with the primary objective to analyze response repertoire of different bee species towards pollen amino acids and their impact upon feeding bees on sugars.