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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)
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ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013
Call For Paper - Volume 12 | Issue 5 | Month- May 2024
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Paper Title
ANTICANCER ACTIVITY OF L- GLUTAMINASE OBTAINED FROM BACILLUS SUBTILIS
Authors
Afshaan Naaz Shaikh Khaleed, R. M. Khobragade
Keywords
L-glutaminase, DPPH, Anticancer, Hep-G2 cell lines, MTT Assay, Bacillus subtilis.
Abstract
This study is set out to explore and purify the L-glutaminase from Bacillus subtilis. Hepatocellular carcinoma has low worldwide prognosis and is one of the most prevalent forms of malignancies. Accumulating data shows the beneficial impact on the occurrence of diverse cancers of amino acid depleting enzymes. Here we investigated the effects of Bacillus subtilis L-glutaminase on Hep-G2 cell lines by in vitro antioxidants and anticancer activity. The enzyme was purified 22.9-fold from a cell-free extract with a final specific activity of 35.71 IU/mg and the recovery was 32.45%. DPPH radical scavenging activity of l-glutaminase IC50 value is 88.34?g/ml. MTT assay reveals IC50 value of L-glutaminase is 161.7?g/ml. These results together suggest that L-glutaminase plays a key function in liver cancer.
IJCRT's Publication Details
Unique Identification Number - IJCRT1133928
Paper ID - 203821
Page Number(s) - 332-339
Pubished in - Volume 6 | Issue 2 | April-2018
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Publisher Name - IJCRT | www.ijcrt.org | ISSN : 2320-2882
E-ISSN Number - 2320-2882
Cite this article
Afshaan Naaz Shaikh Khaleed, R. M. Khobragade, "ANTICANCER ACTIVITY OF L- GLUTAMINASE OBTAINED FROM BACILLUS SUBTILIS", International Journal of Creative Research Thoughts (IJCRT), ISSN:2320-2882, Volume.6, Issue 2, pp.332-339, April-2018, Available at :http://www.ijcrt.org/papers/IJCRT1133928.pdf
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ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
ISSN and 7.97 Impact Factor Details
ISSN: 2320-2882 Impact Factor: 7.97 and ISSN APPROVED Journal Starting Year (ESTD) : 2013
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