ISSN2320-2882
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Paper Title
Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances
Authors
Ms.Bhagyashree B. Wadikar , Ms. Namrata Jayshing Jadhav , Ms. Bhavana Rahul Patil , Mr. Vinod S. Pattankude , Dr. Dhanraj R. Jadge
Keywords
HPLC, Mobile phase selection, Chromatographic optimization, Gradient elution, Buffer systems, Pharmaceutical analysis
Abstract
High-Performance Liquid Chromatography (HPLC) is a widely utilized analytical technique for the separation, identification, and quantification of compounds across pharmaceutical, environmental, and biochemical domains. Among the various factors influencing chromatographic performance, mobile phase selection plays a critical role in determining retention behavior, resolution, selectivity, and peak shape. This review focuses on the fundamental principles governing mobile phase selection, including polarity, pH, buffer systems, organic modifiers, and additives. Different chromatographic modes such as normal phase, reverse phase, ion-exchange, and ion-pair chromatography are discussed in relation to mobile phase characteristics. The article also highlights the impact of mobile phase composition on chromatographic outcomes and outlines key optimization strategies including isocratic and gradient elution, as well as modern approaches like Design of Experiments and AI-assisted method development. Practical aspects such as mobile phase preparation, filtration, and degassing are also addressed. Recent advances including ionic liquids, green chromatography, and UHPLC techniques are reviewed along with current challenges in method development. Overall, this review provides a comprehensive understanding of strategic mobile phase selection for achieving efficient and reproducible HPLC analysis.
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IJCRT — journal style
"Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances", IJCRT - International Journal of Creative Research Thoughts (www.ijcrt.org), ISSN:2320-2882, Vol.14, Issue 4, page no.d695-d705, April 2026, Available :https://ijcrt.org/papers/IJCRT2604438.pdf
APA — 7th edition
Wadikar, M. B. B., Jadhav, N. J., Patil, B. R., Pattankude, V. S., & Jadge, D. R. (2026). Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances. International Journal of Creative Research Thoughts, 14(4), d695-d705. https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438
MLA — 9th edition
Wadikar, Ms.Bhagyashree B., et al. "Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances." International Journal of Creative Research Thoughts, vol. 14, no. 4, 2026, pp. d695-d705, https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438.
Chicago — 17th, bibliography
Wadikar, Ms.Bhagyashree B., et al. "Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances." International Journal of Creative Research Thoughts 14, no. 4 (2026): d695-d705. https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438.
Harvard — author–date
Wadikar, M.B.B. et al. (2026) 'Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances', International Journal of Creative Research Thoughts, 14(4), pp. d695-d705. Available at: https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438
IEEE — numbered reference
M. B. B. Wadikar, N. J. Jadhav, B. R. Patil, V. S. Pattankude and D. R. Jadge, "Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances," IJCRT, vol. 14, no. 4, pp. d695-d705, Apr. 2026.
Vancouver — biomedical
Wadikar MBB, Jadhav NJ, Patil BR, Pattankude VS, Jadge DR. Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances. IJCRT. 2026 Apr;14(4):d695-d705.
AMA — 11th edition
Wadikar MBB, Jadhav NJ, Patil BR, Pattankude VS, Jadge DR. Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances. IJCRT. 2026;14(4):d695-d705. Accessed at https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438
BibTeX — Zotero, Mendeley, LaTeX
@article{IJCRT2604438,
author = {Ms.Bhagyashree B. Wadikar and Namrata Jayshing Jadhav and Bhavana Rahul Patil and Vinod S. Pattankude and Dhanraj R. Jadge},
title = {Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances},
journal = {International Journal of Creative Research Thoughts},
volume = {14},
number = {4},
pages = {d695-d705},
year = {2026},
month = {April},
issn = {2320-2882},
url = {https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - Wadikar, Ms.Bhagyashree B.
AU - Jadhav, Namrata Jayshing
AU - Patil, Bhavana Rahul
AU - Pattankude, Vinod S.
AU - Jadge, Dhanraj R.
TI - Strategic Approaches to Mobile Phase Selection in High-Performance Liquid Chromatography: Principles, Optimization, and Recent Advances
T2 - International Journal of Creative Research Thoughts
JA - IJCRT
VL - 14
IS - 4
PY - 2026
SN - 2320-2882
UR - https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438
SP - d695-d705
ER -
Issue
Volume 14 Issue 4,
April-2026
Pages : d695-d705
Article Information
- Pages
- d695-d705
- Published
- April 2026
- Registration ID
- IJCRT_305466
- Published Paper ID
- IJCRT2604438
- Research Area
- Pharmacy All
- Author Type
- Indian Author
- Location
- Peth vadgaon, maharashtra, India
- Peer Review
- Request Peer Review Report
- Licence
- CC BY 4.0 · Open Access
- ISSN & Impact Factor
- 2320-2882 · IF 9.17
- Downloads
- 147
- Permanent Link
- https://ijcrt.org/viewpaperforall.php?paper=IJCRT2604438
- Published Paper PDF
- https://ijcrt.org/papers/IJCRT2604438.pdf
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