ISSN2320-2882
Impact Factor9.17
UGC ParametersAligned
Low Cost₹1501 / $59
CrossRefDOI
ESTD2013
Peer ReviewTransparent
AccessOpen
Submission: Always Open
Paper Title
Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria
Authors
Arnisha S. Italiya , Dhwani V. Varmora , Riddhi Naresh Dholakiya
Keywords
Actinobacteria, pigment production, extracellular enzymes, amylase, gelatinase, pectinase, TSI test, UV-Vis spectroscopy.
Abstract
Actinobacteria are a diverse group of Gram-positive, filamentous microorganisms known for their remarkable ability to produce industrially valuable enzymes and natural pigments. The present investigation aimed to isolate, characterize, and evaluate pigment-producing Actinobacteria from soil and sediment samples collected from the Kumbharwada wetland region of Bhavnagar, Gujarat, India. Isolation was carried out using Gause's Synthetic Agar(GSA), resulting in a total of 23 anctinobacterial isolates, of which 13 exhibited distinct pigment production. Morphological and microscopic observations confirmed by typical morphological characteristics. Biochemical characterization using the Triple Sugar Iron(TSI) test revealed carbohydrate fermentation without gas or hydrogen sulphide production. Enzymatic screening demonstrated significant extracellular enzyme activity, including amylase, gelatinase and pectinase production, highlighting the biocatalytic potential of the isolates. Pigments were extracted using ethyl acetate and analysed using spectrophotometrically in the range of 400-800nm, revealing diverse absorption maxima indicative of different pigment types. The findings suggest that the Kumbharwada wetland is a rich reservoir of pigment-producing Actionbacteria with potential applications in food, textile, pharmaceutical, and biotechnological industries. The study emphasizes the potential of naturally derived microbial pigments and enzymes as eco-friendly alternatives to synthetic counterparts.
Full Paper, Downloads & Preview
How To Cite
Choose the style your journal or department asks for, then copy it. Every version below is generated from this paper's own record.
IJCRT — journal style
"Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria", IJCRT - International Journal of Creative Research Thoughts (www.ijcrt.org), ISSN:2320-2882, Vol.14, Issue 2, page no.b75-b85, February 2026, Available :https://ijcrt.org/papers/IJCRT2602118.pdf
APA — 7th edition
Italiya, A. S., Varmora, D. V., & Dholakiya, R. N. (2026). Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria. International Journal of Creative Research Thoughts, 14(2), b75-b85. https://doi.org/10.56975/ijcrt.v14i2.300911
MLA — 9th edition
Italiya, Arnisha S., et al. "Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria." International Journal of Creative Research Thoughts, vol. 14, no. 2, 2026, pp. b75-b85, https://doi.org/10.56975/ijcrt.v14i2.300911.
Chicago — 17th, bibliography
Italiya, Arnisha S., Dhwani V. Varmora, and Riddhi Naresh Dholakiya. "Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria." International Journal of Creative Research Thoughts 14, no. 2 (2026): b75-b85. https://doi.org/10.56975/ijcrt.v14i2.300911.
Harvard — author–date
Italiya, A.S., Varmora, D.V. and Dholakiya, R.N. (2026) 'Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria', International Journal of Creative Research Thoughts, 14(2), pp. b75-b85. Available at: https://doi.org/10.56975/ijcrt.v14i2.300911
IEEE — numbered reference
A. S. Italiya, D. V. Varmora and R. N. Dholakiya, "Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria," IJCRT, vol. 14, no. 2, pp. b75-b85, Jan. 2026, doi: 10.56975/ijcrt.v14i2.300911.
Vancouver — biomedical
Italiya AS, Varmora DV, Dholakiya RN. Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria. IJCRT. 2026 Jan;14(2):b75-b85. doi: 10.56975/ijcrt.v14i2.300911
AMA — 11th edition
Italiya AS, Varmora DV, Dholakiya RN. Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria. IJCRT. 2026;14(2):b75-b85. doi:10.56975/ijcrt.v14i2.300911
BibTeX — Zotero, Mendeley, LaTeX
@article{IJCRT2602118,
author = {Arnisha S. Italiya and Dhwani V. Varmora and Riddhi Naresh Dholakiya},
title = {Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria},
journal = {International Journal of Creative Research Thoughts},
volume = {14},
number = {2},
pages = {b75-b85},
year = {2026},
month = {January},
issn = {2320-2882},
doi = {10.56975/ijcrt.v14i2.300911},
url = {https://doi.org/10.56975/ijcrt.v14i2.300911}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - Italiya, Arnisha S.
AU - Varmora, Dhwani V.
AU - Dholakiya, Riddhi Naresh
TI - Isolation, Enzyme Production and Comparison Of Different Pigments From Actinobacteria
T2 - International Journal of Creative Research Thoughts
JA - IJCRT
VL - 14
IS - 2
PY - 2026
SN - 2320-2882
DO - 10.56975/ijcrt.v14i2.300911
UR - https://doi.org/10.56975/ijcrt.v14i2.300911
SP - b75-b85
ER -
Issue
Volume 14 Issue 2,
January-2026
Pages : b75-b85
Article Information
- Pages
- b75-b85
- Published
- January 2026
- Registration ID
- IJCRT_300911
- Published Paper ID
- IJCRT2602118
- Research Area
- Life Sciences All
- Author Type
- Indian Author
- Location
- Bhavnagar, Gujarat, India
- Peer Review
- Request Peer Review Report
- Licence
- CC BY 4.0 · Open Access
- ISSN & Impact Factor
- 2320-2882 · IF 9.17
- Downloads
- 310
- Permanent Link
- https://ijcrt.org/viewpaperforall.php?paper=IJCRT2602118
- Published Paper PDF
- https://ijcrt.org/papers/IJCRT2602118.pdf
Check for updates. Crossmark shows whether this article has been corrected, updated or retracted since publication, and links to the current version of record.
Summarise this Paper with AI
Opens your assistant with a prepared prompt. AI summaries can be incomplete or wrong — read the full paper before citing it.
Author Services
Certificates, printed copies and official reports for this paper
Published authors can request a hard copy of their certificate or of the printed journal, and obtain the transparent peer review and plagiarism reports for this article — each issued against this paper’s registration record.
Registration ID IJCRT_300911Printed copies
Hard Copy of CertificatePrinted certificate for each author, posted to you.
Hard Copy of JournalA bound printed copy of the issue this paper appears in.
Reports & recognition
Transparent Peer Review Report
The reviewer comments and decisions behind this article.
Turnitin Plagiarism Report
The similarity check run on this manuscript.
Best Paper Nomination
Put this article forward for the issue’s best paper award.
Requests are verified against the registration ID of this article.
About Publisher
International Journal of Creative Research Thoughts
Published by IJCRT Publication
2320-2882
ISSN
9.17
Impact Factor
2013
ESTD Year
Open
Access
Impact Factor 9.17, calculated from Google Scholar citation data — see how it is worked out.
An international scholarly open access journal: peer-reviewed, refereed, multidisciplinary, monthly and multilingual, with Crossref DOI registration, rich metadata and a citation generator. Verified indexing & discovery records.
IJCRT is a Transparent, Peer-Reviewed, Open Access Journal (Refereed Journal). Following the discontinuation of UGC and UGC-CARE journal listing, IJCRT is aligned with UGC’s Suggested Parameters for Choosing Peer-Reviewed Journals. UGC Public Notice
Licence
© 2026 — Authors hold the copyright of this article.
This work is licensed under a
Creative Commons Attribution 4.0 International License
and The Open Definition.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0),
which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Disclaimer:
The content, data and findings in this article are based on the authors’ research and have been
peer-reviewed for academic purposes only. Readers are advised to verify all information before practical
or commercial use. The journal and its editorial board are not liable for any errors, losses or
consequences arising from its use.
Declarations
Funding
No external funding was received for this study.
Conflict of Interest
The authors declare that they have no conflict of interest.
Acknowledgements
The authors would like to thank the reviewers and the editorial board of International Journal of Creative Research Thoughts for their careful reading and constructive comments, and all colleagues who supported the preparation of this manuscript.
About the Journal
9.17Impact Factor
UGC AlignedSuggested Parameters
TransparentPeer Review
Open AccessNo Paywall
CrossrefDOI Available
Monthly12 Issues / Year
Since 2013Established
2320-2882ISSN
How Publication Works
1Submit Your PaperOnline or by email. A registration ID is issued straight away.
2Transparent Peer ReviewDouble-blind review by subject experts, reported openly.
3AcceptanceThe result reaches you by email and SMS against your registration ID.
4Published Open AccessFree to read worldwide, with certificate and letter ready to download.
Article Impact
51Views
50Downloads
101Total reads
Jan 2026Published
Published · 252 days ago
Today · last read today
2.4 views and 2.4 downloads per day over 21 days counted.
Counts exclude identified crawler traffic and repeat hits from the same reader within 30 minutes. Downloads reached directly through a search engine or an existing citation link are not included, so the figure is a conservative count of activity through this site. Full statistics
Discovery & Indexing
Find this paper on search engines and scholarly indexes, and check the journal’s verified records.
Find this paperSearch
Google ScholarExact-title search, with citing articles once indexed.Search
CrossrefThe registered DOI record and its metadata.Open DOI record
Semantic ScholarAI-powered literature search.Search- BASEBASEBielefeld Academic Search Engine — open-access harvester.Search
ResearchGateResearch network publication search.Search
Journal recordsVerified
“Search” opens a live search for this paper; “Verified” opens a journal record checked on the Indexing & Discovery page. Third-party services index at their own discretion, often several weeks after publication.
