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Paper Title
Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis
Authors
Ch. Thirupathi , P. Muthenna , Anugula Chandra Shekhar
Keywords
Protein metabolism, nucleic acid synthesis, amino acid catabolism, glucose homeostasis, metabolic reprogramming
Abstract
Dietary organic acids significantly influence fish metabolism through complex biochemical pathways affecting protein synthesis, amino acid metabolism, and nucleic acid dynamics. This study investigated the temporal effects of graded citric acid supplementation (2%, 4%, and 6%) on tissue-specific protein content, free amino acid profiles, nucleic acid concentrations, and blood glucose levels in Channa striatus over 28 days. Fish were fed experimental diets and sampled at 7, 14, 21, and 28 days for biochemical analysis of muscle and liver tissues. Protein quantification utilized Bradford assay, amino acids were analyzed by ninhydrin method, and nucleic acids were extracted and quantified spectrophotometrically. Results revealed significant dose-dependent increases in tissue protein content, with 6% citric acid treatment achieving 84.86% and 49.14% increases in muscle and liver proteins, respectively, after 28 days. Conversely, free amino acid levels decreased progressively, indicating enhanced incorporation into protein synthesis pathways. RNA content increased substantially in both tissues (23.05% in muscle, 40.05% in liver with 6% treatment), while DNA showed modest but significant increases at higher doses. Blood glucose levels decreased significantly across all treatments, suggesting improved glucose utilization efficiency. These findings demonstrate that citric acid supplementation orchestrates a comprehensive metabolic reprogramming characterized by enhanced protein anabolism, accelerated nucleic acid synthesis, and optimized glucose metabolism, providing mechanistic insights into growth enhancement in aquaculture species.
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IJCRT — journal style
"Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis", IJCRT - International Journal of Creative Research Thoughts (www.ijcrt.org), ISSN:2320-2882, Vol.13, Issue 9, page no.a46-a67, September 2025, Available :https://ijcrt.org/papers/IJCRT2509007.pdf
APA — 7th edition
Thirupathi, C., Muthenna, P., & Shekhar, A. C. (2025). Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis. International Journal of Creative Research Thoughts, 13(9), a46-a67. https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007
MLA — 9th edition
Thirupathi, Ch., et al. "Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis." International Journal of Creative Research Thoughts, vol. 13, no. 9, 2025, pp. a46-a67, https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007.
Chicago — 17th, bibliography
Thirupathi, Ch., P. Muthenna, and Anugula Chandra Shekhar. "Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis." International Journal of Creative Research Thoughts 13, no. 9 (2025): a46-a67. https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007.
Harvard — author–date
Thirupathi, C., Muthenna, P. and Shekhar, A.C. (2025) 'Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis', International Journal of Creative Research Thoughts, 13(9), pp. a46-a67. Available at: https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007
IEEE — numbered reference
C. Thirupathi, P. Muthenna and A. C. Shekhar, "Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis," IJCRT, vol. 13, no. 9, pp. a46-a67, Sep. 2025.
Vancouver — biomedical
Thirupathi C, Muthenna P, Shekhar AC. Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis. IJCRT. 2025 Sep;13(9):a46-a67.
AMA — 11th edition
Thirupathi C, Muthenna P, Shekhar AC. Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis. IJCRT. 2025;13(9):a46-a67. Accessed at https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007
BibTeX — Zotero, Mendeley, LaTeX
@article{IJCRT2509007,
author = {Ch. Thirupathi and P. Muthenna and Anugula Chandra Shekhar},
title = {Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis},
journal = {International Journal of Creative Research Thoughts},
volume = {13},
number = {9},
pages = {a46-a67},
year = {2025},
month = {September},
issn = {2320-2882},
url = {https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - Thirupathi, Ch.
AU - Muthenna, P.
AU - Shekhar, Anugula Chandra
TI - Citric Acid-Induced Modulation of Protein Metabolism and Nucleic Acid Dynamics in Channa striatus: Insights into Metabolic Reprogramming and Cellular Biosynthesis
T2 - International Journal of Creative Research Thoughts
JA - IJCRT
VL - 13
IS - 9
PY - 2025
SN - 2320-2882
UR - https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007
SP - a46-a67
ER -
Issue
Volume 13 Issue 9,
September-2025
Pages : a46-a67
Article Information
- Published In
- International Journal of Creative Research Thoughts — Volume 13, Issue 9, September 2025
- Pages
- a46-a67
- Published
- September 2025
- Registration ID
- IJCRT_293315
- Published Paper ID
- IJCRT2509007
- Research Area
- Science and Technology
- Author Type
- Indian Author
- Location
- India
- Peer Review
- Request Peer Review Report
- Licence
- CC BY 4.0 · Open Access
- ISSN & Impact Factor
- 2320-2882 · IF 9.17
- Downloads
- 340
- Permanent Link
- https://ijcrt.org/viewpaperforall.php?paper=IJCRT2509007
- Published Paper PDF
- https://ijcrt.org/papers/IJCRT2509007.pdf
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