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Paper Title
Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches
Authors
Ashish B. Kalasava , Kruti G. Dangar , Dr. Geera P. Mankad
Keywords
Halophilic actinomycetes; Actinopolyspora erythraea; Amylases; Bioinformatics analysis; Protein structure prediction
Abstract
Halophilic actinomycetes are valuable sources of extremozymes with significant industrial potential due to their Sustain ability and function under high salinity and other harsh conditions. In this study, we conducted comprehensive bioinformatics-based structural and functional analysis on four amylase sequences from the halophilic actinomycetes Actinopolyspora erythraea, including ?-glucosidase, glycosidase and two hypothetical proteins. We retrieved four amino acid sequences from the NCBI database. Sequences were analysed by using ProtParam to determine physicochemical properties associated with halophilic adaptation. Three-dimensional structures were predicted using SWISS-MODEL and validated through Global Model Quality Estimation (GMQE), QMEAN Z-score, MolProbity and Ramachandran plot to capture the novelty of amylases.
All analysed proteins exhibited hallmark features of halophilic enzymes, including low theoretical isoelectric points, enrichment of acidic amino acids, negative GRAVY values indicating enhanced hydrophilicity, and moderate to high aliphatic indices suggestive of thermostability. The ?-glucosidase and glycosidase proteins demonstrated favourable stability indices and structural robustness, supporting their primary roles in carbohydrate metabolism under hypersaline conditions.
Overall, this study highlights key molecular determinants underlying halophilic adaptation in amylase-related proteins from Actinopolyspora erythraea and underscores the biotechnological potential of halophilic actinomycetes as promising sources of salt-tolerant enzymes for applications in the food, detergent, and pharmaceutical industries.
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IJCRT — journal style
"Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches", IJCRT - International Journal of Creative Research Thoughts (www.ijcrt.org), ISSN:2320-2882, Vol.7, Issue 1, page no.427-433, January 2019, Available :https://ijcrt.org/papers/IJCRT1136201.pdf
APA — 7th edition
Kalasava, A. B., Dangar, K. G., & Mankad, G. P. (2019). Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches. International Journal of Creative Research Thoughts, 7(1), 427-433. https://doi.org/10.56975/ijcrt.v7i1.299907
MLA — 9th edition
Kalasava, Ashish B., et al. "Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches." International Journal of Creative Research Thoughts, vol. 7, no. 1, 2019, pp. 427-433, https://doi.org/10.56975/ijcrt.v7i1.299907.
Chicago — 17th, bibliography
Kalasava, Ashish B., Kruti G. Dangar, and Geera P. Mankad. "Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches." International Journal of Creative Research Thoughts 7, no. 1 (2019): 427-433. https://doi.org/10.56975/ijcrt.v7i1.299907.
Harvard — author–date
Kalasava, A.B., Dangar, K.G. and Mankad, G.P. (2019) 'Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches', International Journal of Creative Research Thoughts, 7(1), pp. 427-433. Available at: https://doi.org/10.56975/ijcrt.v7i1.299907
IEEE — numbered reference
A. B. Kalasava, K. G. Dangar and G. P. Mankad, "Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches," IJCRT, vol. 7, no. 1, pp. 427-433, Jan. 2019, doi: 10.56975/ijcrt.v7i1.299907.
Vancouver — biomedical
Kalasava AB, Dangar KG, Mankad GP. Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches. IJCRT. 2019 Jan;7(1):427-433. doi: 10.56975/ijcrt.v7i1.299907
AMA — 11th edition
Kalasava AB, Dangar KG, Mankad GP. Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches. IJCRT. 2019;7(1):427-433. doi:10.56975/ijcrt.v7i1.299907
BibTeX — Zotero, Mendeley, LaTeX
@article{IJCRT1136201,
author = {Ashish B. Kalasava and Kruti G. Dangar and Geera P. Mankad},
title = {Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches},
journal = {International Journal of Creative Research Thoughts},
volume = {7},
number = {1},
pages = {427-433},
year = {2019},
month = {January},
issn = {2320-2882},
doi = {10.56975/ijcrt.v7i1.299907},
url = {https://doi.org/10.56975/ijcrt.v7i1.299907}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - Kalasava, Ashish B.
AU - Dangar, Kruti G.
AU - Mankad, Geera P.
TI - Structural and functional sequence analysis of amylase - producing halophilic actinomycetes using bioinformatics approaches
T2 - International Journal of Creative Research Thoughts
JA - IJCRT
VL - 7
IS - 1
PY - 2019
SN - 2320-2882
DO - 10.56975/ijcrt.v7i1.299907
UR - https://doi.org/10.56975/ijcrt.v7i1.299907
SP - 427
EP - 433
ER -
Issue
Volume 7 Issue 1,
January-2019
Pages : 427-433
Article Information
- Pages
- 427-433
- Published
- January 2019
- Registration ID
- IJCRT_299907
- Published Paper ID
- IJCRT1136201
- Research Area
- Life Sciences All
- Author Type
- Indian Author
- Location
- Rajkot, Gujarat, India
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- Published Paper PDF
- https://ijcrt.org/papers/IJCRT1136201.pdf
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